期刊
MARINE GENOMICS
卷 59, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.margen.2021.100864
关键词
Archaea; Illumina; NGS; Marine; Methane; Methanogenesis
资金
- Science and Engineering Research Board of Department of Science and Technology (SERB-DST)(Government of India, New Delhi, India) [YSS/2015/001826]
- Agharkar Research Institute, Pune, Maharashtra, India
Methanosarcina sp. strain MSH10X1 is a psychrotrophic methanogen isolated from sub-seafloor methane hydrate deposits in the Krishna Godavari Basin. It is capable of utilizing various substrates for methanogenesis reactions, and genomic analysis suggests its adaptation to low-temperature conditions in the deep sea.
Methanosarcina sp. strain MSH10X1, a psychrotrophic methanogen, was isolated from sub-seafloor methane hydrate deposits of Krishna Godavari Basin on India's east coast. The strain could grow from 5 to 40 degrees C following all three i.e. methylotrophic, acetoclastic, and hydrogenotrophic modes of methanogenesis utilizing different substrates like methanol, trimethylamine, H2/CO2 (80/20), acetate, valerate, isobutyrate, isopropanol, and isobutanol. The genome sequencing and analysis of this strain revealed a circular chromosome of 3,557,383 bp length having 42.47 mol% G + C content, which consisted of 3110 coding genes, 58 tRNA genes, and 3 rRNA operons. The KEGG analysis highlighted the presence of genes responsible for all three modes of methanogenesis. The presence of genes like mtaB, mtaC, and mttB in the genome provided evidence for possible adaptation of strain MSH10X1 in the deep sea's low-temperature conditions.
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