期刊
MARINE DRUGS
卷 19, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/md19030171
关键词
exopolysaccharide; antitumor; FGF19-FGFR4 signaling; cell growth; cell adhesion
资金
- China Ocean Mineral Resources R&D Association Grant [DY295-B2-14]
- National Key R and D Program of China [2018YFC0310800]
- Taishan Young Scholar Program of Shandong Province [tsqn20161051]
- Qingdao Innovation Leadership Program [18-1-2-7-zhc]
- Natural Science Foundation of Shandong Province [ZR2019BH024]
- National Natural Science Foundation of China [42006081]
- Basic Applied Research program of Qingdao [19-6-2-35-cg]
EPS364, a novel exopolysaccharide purified from Vibrio alginolyticus 364, displayed significant antitumor activity in liver cancer cells by inducing apoptosis, disrupting MMP, and generating ROS, possibly through targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.
The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a beta-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.
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