期刊
MARINE DRUGS
卷 19, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/md19050254
关键词
Solea senegalensis; Fads2; Elovl5; LC-PUFA biosynthesis; diet; salinity; EPA; DHA
资金
- project PROPUFAW3 of the Ministry of Science, Innovation and Universities, Spanish Government [AGL2015-70994-R]
- Agencia Canaria de Investigacion, Innovacion y Sociedad de la Informacion de la Consejeria de Economia, Conocimiento y Empleo
- Fondo Social Europeo (FSE) Programa Operativo Integrado de Canarias 2014-2020, Eje 3 Tema Prioritario 74 (85%)
- CajaSiete
This study aims to evaluate the combined effects of dietary composition and salinity on the fatty acid composition and enzymatic activity in different body compartments of fish. The findings show that dietary composition primarily regulates the expression of fads2, while salinity plays a stronger role in regulating elovl5.
Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA) depending upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. It has been reported in Solea senegalensis the existence of a Delta 4 desaturase, enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), which can be modulated by the diet. The present study aims to evaluate the combined effects of the partial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in the fatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), the enzymatic activity over alpha-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation of isolated hepatocytes and enterocytes with [1-C-14] 18:3 n-3, and the regulation of the S. senegalensis fads2 and elovl5 in the liver and intestine. The presence of radiolabelled products, including 18:4n-3, 20:4n-3 and EPA, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA from ALA, establishing S. senegalensis, has at least one Fads2 with increment 6 activity. Dietary composition prevailed over salinity in regulating the expression of fads2, while salinity did so over dietary composition for elovl5. FO replacement enhanced the proportion of DHA in S. senegalensis muscle and the combination with 20 ppt salinity increased the amount of n-3 LC-PUFA in hepatocytes.
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