4.6 Article

Structural Analysis of the OC43 Coronavirus 2′-O-RNA Methyltransferase

期刊

JOURNAL OF VIROLOGY
卷 95, 期 15, 页码 -

出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00463-21

关键词

OC43; coronavirus; crystal structure; methyltransferase

类别

资金

  1. Czech Science Foundation [21-25280S]
  2. Academy of Sciences of the Czech Republic [RVO 61388963]

向作者/读者索取更多资源

The crystal structure of 29-O-RNA methyltransferase of the OC43 coronavirus was reported, showing both similarities and differences in comparison with other coronaviral enzymes. The conservation of the SAM binding site suggests the potential for designing antivirals effective against multiple human coronaviruses.
The OC43 coronavirus is a human pathogen that usually causes only the common cold. One of its key enzymes, similar to other coronaviruses, is the 29-O-RNA methyltransferase (MTase), which is essential for viral RNA stability and expression. Here, we report the crystal structure of the 29-O-RNA MTase in a complex with the pan-methyltransferase inhibitor sinefungin solved at 2.2-A resolution. The structure reveals an overall fold consistent with the fold observed in other coronaviral MTases. The major differences are in the conformation of the C terminus of the nsp16 subunit and an additional helix in the N terminus of the nsp10 subunits. The structural analysis also revealed very high conservation of the S-adenosyl methionine (SAM) binding pocket, suggesting that the SAM pocket is a suitable spot for the design of antivirals effective against all human coronaviruses. IMPORTANCE Some coronaviruses are dangerous pathogens, while some cause only common colds. The reasons are not understood, although the spike proteins probably play an important role. However, to understand the coronaviral biology in sufficient detail, we need to compare the key enzymes from different coronaviruses. We solved the crystal structure of 29-O-RNA methyltransferase of the OC43 coronavirus, a virus that usually causes mild colds. The structure revealed some differences in the overall fold but also revealed that the SAM binding site is conserved, suggesting that development of antivirals against multiple coronaviruses is feasible.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据