期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 143, 期 17, 页码 6560-6577出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.1c01180
关键词
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资金
- Max Planck Society
This study extensively simulated possible conformations of intermediates in the sMMOH catalytic cycle using a quantum mechanics/molecular mechanics approach, providing strong support for an open-core configuration in MMOHQ. The implications of this open-core intermediate Q on the reaction mechanism of sMMO are discussed.
The determination of the diiron core intermediate structures involved in the catalytic cycle of soluble methane monooxygenase (sMMO), the enzyme that selectively catalyzes the conversion of methane to methanol, has been a subject of intense interest within the bioinorganic scientific community. Particularly, the specific geometry and electronic structure of the intermediate that precedes methane binding, known as intermediate Q (or MMOHQ), has been debated for over 30 years. Some reported studies support a bis-mu-oxo-bridged Fe(IV)(2)O-2 closed-core conformation Fe(IV)(2)O-2 core, whereas others favor an open-core geometry, with a longer Fe-Fe distance. The lack of consensus calls for a thorough re-examination and reinterpretation of the spectroscopic data available on the MMOHQ intermediate. Herein, we report extensive simulations based on a hybrid quantum mechanics/molecular mechanics approach (QM/MM) approach that takes into account the complete enzyme to explore possible conformations for intermediates MMOHox and MMOHQ of the sMMOH catalytic cycle. High-level quantum chemical approaches are used to correlate specific structural motifs with geometric parameters for comparison with crystallographic and EXAFS data, as well as with spectroscopic data from Mossbauer spectroscopy, Fe K-edge high-energy resolution X-ray absorption spectroscopy (HERFD XAS), and resonance Raman O-16-O-18 difference spectroscopy. The results provide strong support for an open-core-type configuration in MMOHQ, with the most likely topology involving mono-oxo-bridged Fe ions and alternate terminal Fe-oxo and Fe-hydroxo groups that interact via intramolecular hydrogen bonding. The implications of an open-core intermediate Q on the reaction mechanism of sMMO are discussed.
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