4.8 Article

Modeling the Catalytic Cycle of Glutathione Peroxidase by Nuclear Magnetic Resonance Spectroscopic Analysis of Selenocysteine Selenenic Acids

期刊

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 143, 期 17, 页码 6345-6350

出版社

AMER CHEMICAL SOC
DOI: 10.1021/jacs.1c02383

关键词

-

资金

  1. Ministry of Education, Culture, Sports, Science, and Technology (Japan) [15H03776, 19H02698]
  2. Grants-in-Aid for Scientific Research [19H02698, 15H03776] Funding Source: KAKEN

向作者/读者索取更多资源

This study provides the first direct spectroscopic evidence for the formation of Sec-SeOH in small-molecule selenocysteine and selenopeptide model systems. The catalytic cycle of glutathione peroxidase was investigated using NMR-observable Sec-SeOH models, examining various chemical processes including intramolecular cyclization.
Although selenocysteine selenenic acids (Sec-SeOHs) have been recognized as key intermediates in the catalytic cycle of glutathione peroxidase (GPx), examples of the direct observation of Sec-SeOH in either protein or small-molecule systems have remained elusive so far, mostly due to their instability. Here, we report the first direct spectroscopic (H-1 and Se-77 NMR) evidence for the formation of Sec-SeOH in small-molecule selenocysteine and selenopeptide model systems with a cradle-type protective group. The catalytic cycle of GPx was investigated using NMR-observable Sec-SeOH models. All the hitherto proposed chemical processes, i.e., not only those of the canonical catalytic cycle but also those involved in the bypass mechanism, including the intramolecular cyclization of Sec-SeOH to the corresponding five-membered ring selenenyl amide, were examined in a stepwise manner.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据