期刊
JOURNAL OF SURGICAL RESEARCH
卷 260, 期 -, 页码 315-324出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2020.11.084
关键词
Trauma; HMGB1; G-CSF; VCAM-1; Neutrophil elastase
类别
资金
- National Institute of General Medical Sciences, United States (NIGMS) [R01 GM105893-01A1, R01 GM113945-01, P50 GM111152-01]
- NIGMS [T32 GM-008721]
- NIH Heart, Lung, and Blood Training, United States [T35HL007489]
This study showed that propranolol could reduce the expression of HMGB1, G-CSF, and neutrophil elastase, resulting in a decreased mobilization of hematopoietic progenitor cells in response to chronic stress and trauma.
Background: This study investigated the molecular mediators of prolonged hematopoietic progenitor cell mobilization a trauma and chronic stress and the role of propranolol in modifying this response. Methods: Sprague-Dawley rats were randomized to lung contusion (LC), LC plus hemorrhagic shock (LCHS), or LCHS with daily restraint stress (LCHS/CS). Propranolol was administered daily. Bone marrow (BM) and lung expression of high mobility group box 1 (HMGB1), granulocyte colony-stimulating factor (G-CSF), neutrophil elastase, stromal cell-derived factor 1 (SDF-1)/CXR4, and vascular cell adhesion protein 1 (VCAM-1)/very late antigen-4 were measured by real-time polymerase chain reaction. Results: Bone marrow HMGB1, G-CSF, and neutrophil elastase expression were significantly elevated two-to four-fold after LCHS/CS, and all were decreased with the use of propranolol. SDF-1 and VCAM-1 were both significantly decreased after LCHS/CS. Conclusions: The increased expression of HMGB1 and G-CSF and decreased expression of BM anchoring molecules, SDF-1 and VCAM-1, after LCHS/CS, likely mediates prolonged hematopoietic progenitor cell mobilization. Propranolol's ability to reduce HMGB1, G-CSF, and neutrophil elastase expression suggests that the mobilization of hematopoietic progenitor cells was driven by persistent hypercatecholaminemia. (c) 2020 Elsevier Inc. All rights reserved.
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