期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 46, 期 11, 页码 2659-2668出版社
WILEY-BLACKWELL
DOI: 10.1002/eji.201646359
关键词
Antigen presentation; Antigen transfer; Delayed-type hypersensitivity; Intralymphatic migration; Resting pDCs; Tolerance; Two-photon imaging
类别
资金
- DFG [SFB738-B3]
- ERC [322645-LYMPHATICS-HOMING]
Plasmacytoid dendritic cells (pDCs) have been shown to induce tolerance to innocuous antigens. Their migratory properties allow them to take up antigens from the periphery and transport them to the draining lymph nodes or to the thymus. However, pDC-T-cell interaction in the primary and secondary lymphoid organs still remains poorly defined. In this study, we show that resting pDCs loaded with exogenous antigen could induce tolerance when transferred intralymphatically into a single lymph node of wild-type C57BL/6 mice. However, this was a result of antigen transfer from pDCs to endogenous antigen presenting cells and subsequent abortive proliferation of cognate CD4(+) T cells. pDCs could not directly induce the proliferation of CD4(+) T cells, as observed in mice lacking MHC class II gene. Moreover, pDCs failed to make physical contacts with OT-II cells as revealed by two-photon imaging. Thus, the role of resting pDCs in tolerance induction seems to be independent of its direct interaction with cognate CD4(+) T cells.
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