期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 260, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2021.153395
关键词
Oryza sativa; Antisense; Rubisco; RBCS; C-4 rice
资金
- Bill & Melinda Gates Foundation [51586]
This study utilized antisense constructs targeting two of the five Rubisco small subunit genes to reduce Rubisco protein content in leaves, limiting CO2 assimilation rate and resulting in a significant decrease in dry biomass accumulation and grain yield. The results suggest that knocking down multiple genes is necessary to completely reduce Rubisco accumulation in MCs.
We generated antisense constructs targeting two of the five Rubisco small subunit genes (OsRBCS2 and 4) which account for between 30-40 % of the RBCS transcript abundance in leaf blades. The constructs were driven by a maize phosphoenolpyruvate carboxylase (PEPC) promoter known to have enriched expression in mesophyll cells (MCs). In the resulting lines leaf, Rubisco protein content was reduced by between 30-50 % and CO2 assimilation rate was limited under photorespiratory and non-photorespiratory conditions. A relationship between Rubisco protein content and CO2 assimilation rate was found. This was associated with a significant reduction in dry biomass accumulation and grain yield of between 37-70%. In addition to serving as a resource for reducing Rubisco accumulation in a cell-preferential manner, these lines allow us to characterize gene function and isoform specific suppression on photosynthesis and growth. Our results suggest that the knockdown of multiple genes is required to completely reduce Rubisco accumulation in MCs.
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