4.5 Article

Cleavage-Resistant Protein Labeling With Hydrophilic Trityl Enables Distance Measurements In-Cell

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Review Chemistry, Multidisciplinary

Going the dHis-tance: Site-Directed Cu2+ Labeling of Proteins and Nucleic Acids

Austin Gamble Jarvi et al.

Summary: This Account showcases the use of site-directed Cu2+ labeling in proteins and DNA for measuring biomolecular structure and dynamics with electron paramagnetic resonance (EPR) spectroscopy. The labeling scheme provides a straightforward method for obtaining biophysical information that is not accessible using traditional EPR labels. The integration of molecular dynamics (MD) simulations with EPR distance information is expected to advance our understanding of protein and DNA conformational changes and interactions.

ACCOUNTS OF CHEMICAL RESEARCH (2021)

Article Chemistry, Multidisciplinary

Ox-SLIM: Synthesis of and Site-Specific Labelling with a Highly Hydrophilic Trityl Spin Label

Nico Fleck et al.

Summary: The highly hydrophilic trityl spin label Ox-SLIM overcomes the issue of aggregation with proteins and unsuitable bioconjugation groups, enabling highly sensitive PDS measurements of protein interspin distances within cells down to concentrations as low as 45 nm.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

Article Chemistry, Multidisciplinary

In Situ Labeling and Distance Measurements of Membrane Proteins in E. coli Using Finland and OX063 Trityl Labels

Sophie Ketter et al.

Summary: By utilizing novel labels, efficient in situ labeling of membrane proteins BtuB and BamA can be achieved, eliminating background signals, enhancing measurement sensitivity and accuracy, deepening our understanding of the conformations of membrane proteins in the cellular environment of E. coli.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

Article Chemistry, Multidisciplinary

Methanethiosulfonate Derivative of OX063 Trityl: A Promising and Efficient Reagent for Side-Directed Spin Labeling of Proteins

Victor M. Tormyshev et al.

CHEMISTRY-A EUROPEAN JOURNAL (2020)

Article Chemistry, Physical

In-Cell Trityl-Trityl Distance Measurements on Proteins

Yin Yang et al.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2020)

Article Chemistry, Multidisciplinary

SLIM: A Short-Linked, Highly Redox-Stable Trityl Label for High-Sensitivity In-Cell EPR Distance Measurements

Nico Fleck et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Chemistry, Multidisciplinary

Double Histidine Based EPR Measurements at Physiological Temperatures Permit Site-Specific Elucidation of Hidden Dynamics in Enzymes

Kevin Singewald et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Multidisciplinary Sciences

In-cell destabilization of a homodimeric protein complex detected by DEER spectroscopy

Yin Yang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Chemistry, Physical

Combining site-directed spin labeling in vivo and in-cell EPR distance determination

Pia Widder et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2020)

Article Multidisciplinary Sciences

Quantifying protein dynamics and stability in a living organism

Ruopei Feng et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

Protein Spin Labeling with a Photocaged Nitroxide Using Diels-Alder Chemistry

Anandi Kugele et al.

CHEMBIOCHEM (2019)

Article Chemistry, Multidisciplinary

Effects of Macromolecular Crowding on Biochemical Systems

Todd P. Silverstein et al.

JOURNAL OF CHEMICAL EDUCATION (2019)

Article Chemistry, Multidisciplinary

Pulsed EPR Dipolar Spectroscopy on Spin Pairs with one Highly Anisotropic Spin Center: The Low-Spin FeIII Case

Dinar Abdullin et al.

CHEMISTRY-A EUROPEAN JOURNAL (2019)

Article Chemistry, Multidisciplinary

EPR Spectroscopy Detects Various Active State Conformations of the Transcriptional Regulator CueR

Hila Sameach et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

Article Chemistry, Physical

Efficient localization of a native metal ion within a protein by Cu2+-based EPR distance measurements

Austin Gamble Jarvi et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2019)

Article Chemistry, Multidisciplinary

Synthesis of hydroxyethyl tetrathiatriarylmethyl radicals OX063 and OX071

Martin Poncelet et al.

RSC ADVANCES (2019)

Article Chemistry, Physical

DEER distance measurements on trityl/trityl and Gd(III)/trityl labelled proteins

Angeliki Giannoulis et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2019)

Article Chemistry, Multidisciplinary

A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies

Ganesan Karthikeyan et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Chemistry, Physical

Room-temperature distance measurements using RIDME and the orthogonal spin labels trityl/nitroxide

Andrey A. Kuzhelev et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2018)

Article Chemistry, Multidisciplinary

In-Cell Titration of Small Solutes Controls Protein Stability and Aggregation

Shahar Sukenik et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

Article Biochemistry & Molecular Biology

Light-Driven Domain Mechanics of a Minimal Phytochrome Photosensory Module Studied by EPR

Tufa E. Assafa et al.

STRUCTURE (2018)

Article Multidisciplinary Sciences

Protein shape modulates crowding effects

Alex J. Guseman et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Article Biochemistry & Molecular Biology

Towards developing principles of protein folding and dynamics in the cell

Margaret S. Cheung et al.

PHYSICAL BIOLOGY (2018)

Article Chemistry, Multidisciplinary

Versatile Trityl Spin Labels for Nanometer Distance Measurements on Biomolecules In Vitro and within Cells

J. Jacques Jassoy et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Article Biochemical Research Methods

Orthogonal spin labeling using click chemistry for in vitro and in vivo applications

Svetlana Kucher et al.

JOURNAL OF MAGNETIC RESONANCE (2017)

Article Chemistry, Multidisciplinary

Studying the Conformation of a Receptor Tyrosine Kinase in Solution by Inhibitor-Based Spin Labeling

Dongsheng M. Yin et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Article Chemistry, Multidisciplinary

Using Genetically Encodable Self-Assembling GdIII Spin Labels To Make In-Cell Nanometric Distance Measurements

Florencia C. Mascali et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2016)

Article Chemistry, Multidisciplinary

Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR

Benesh Joseph et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2016)

Article Biochemistry & Molecular Biology

Calcineurin in a Crowded World

Erik C. Cook et al.

BIOCHEMISTRY (2016)

Article Chemistry, Multidisciplinary

Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR

Silvia Valera et al.

CHEMISTRY-A EUROPEAN JOURNAL (2016)

Review Chemistry, Organic

Site-directed spin labeling of proteins for distance measurements in vitro and in cells

P. Roser et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2016)

Article Chemistry, Physical

Paramagnetic spherical nanoparticles by the self-assembly of persistent trityl radicals

I. Marin-Montesinos et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2016)

Article Chemistry, Physical

Electron spin dynamics and spin-lattice relaxation of trityl radicals in frozen solutions

Hanjiao Chen et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2016)

Article Chemistry, Physical

Pulsed EPR characterization of HIV-1 protease conformational sampling and inhibitor-induced population shifts

Zhanglong Liu et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2016)

Article Chemistry, Physical

Flexibility and conformation of the cocaine aptamer studied by PELDOR

C. M. Grytz et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2016)

Article Biochemistry & Molecular Biology

Munc18-1 and the Syntaxin-1 N Terminus Regulate Open-Closed States in a t-SNARE Complex

Damian Dawidowski et al.

STRUCTURE (2016)

Article Biochemistry & Molecular Biology

EPR Distance Measurements in Native Proteins with Genetically Encoded Spin Labels

Moritz J. Schmidt et al.

ACS CHEMICAL BIOLOGY (2015)

Article Chemistry, Multidisciplinary

Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

Filip Wojciechowski et al.

CHEMICAL COMMUNICATIONS (2015)

Article Chemistry, Physical

Triarylmethyl Labels: Toward Improving the Accuracy of EPR Nanoscale Distance Measurements in DNAs

Georgiy Yu. Shevelev et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2015)

Article Chemistry, Physical

Room-Temperature Electron Spin Relaxation of Triarylmethyl Radicals at the X- and Q-Bands

Andrey A. Kuzhelev et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2015)

Article Biochemical Research Methods

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells

Iwo Koenig et al.

NATURE METHODS (2015)

Article Biochemistry & Molecular Biology

Structural Mechanism for the Regulation of HCN Ion Channels by the Accessory Protein TRIP8b

Hannah A. DeBerg et al.

STRUCTURE (2015)

Article Chemistry, Multidisciplinary

EPR-Based Approach for the Localization of Paramagnetic Metal Ions in Biomolecules

Dinar Abdullin et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

Article Chemistry, Multidisciplinary

Gd(III)-PyMTA Label Is Suitable for In-Cell EPR

Mian Qi et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Article Chemistry, Multidisciplinary

Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy

Georgiy Yu. Shevelev et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Article Chemistry, Multidisciplinary

Probing Protein Conformation in Cells by EPR Distance Measurements using Gd3+ Spin Labeling

Andrea Martorana et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Article Chemistry, Multidisciplinary

Protein Stabilization by Macromolecular Crowding through Enthalpy Rather Than Entropy

Michael Senske et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Article Chemistry, Physical

Gd3+ spin labeling for distance measurements by pulse EPR spectroscopy

Daniella Goldfarb

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2014)

Article Multidisciplinary Sciences

Molecular mechanism of Mg2+-dependent gating in CorA

Olivier Dalmas et al.

NATURE COMMUNICATIONS (2014)

Article Biochemistry & Molecular Biology

Technological advances in site-directed spin labeling of proteins

Wayne L. Hubbell et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2013)

Article Chemistry, Organic

Esterified Dendritic TAM Radicals with Very High Stability and Enhanced Oxygen Sensitivity

Yuguang Song et al.

JOURNAL OF ORGANIC CHEMISTRY (2013)

Article Chemistry, Multidisciplinary

Phosphonated Trityl Probes for Concurrent in Vivo Tissue Oxygen and pH Monitoring Using Electron Paramagnetic Resonance-Based Techniques

Ilirian Dhimitruka et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Biochemistry & Molecular Biology

Conformational heterogeneity of the aspartate transporter GltPh

Inga Hanelt et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2013)

Article Physics, Atomic, Molecular & Chemical

MtsslWizard: In Silico Spin-Labeling and Generation of Distance Distributions in PyMOL

Gregor Hagelueken et al.

APPLIED MAGNETIC RESONANCE (2012)

Article Chemistry, Multidisciplinary

Trityl Radicals: Spin Labels for Nanometer-Distance Measurements

Gunnar W. Reginsson et al.

CHEMISTRY-A EUROPEAN JOURNAL (2012)

Article Chemistry, Multidisciplinary

Pulsed ESR Dipolar Spectroscopy for Distance Measurements in Immobilized Spin Labeled Proteins in Liquid Solution

Zhongyu Yang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)

Article Chemistry, Organic

Synthesis and Reduction Kinetics of Sterically Shielded Pyrrolidine Nitroxides

Joseph T. Paletta et al.

ORGANIC LETTERS (2012)

Article Chemistry, Multidisciplinary

Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

Ivan Krstic et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)

Article Biochemical Research Methods

Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes

Mykhailo Azarkh et al.

JOURNAL OF MAGNETIC RESONANCE (2011)

Article Chemistry, Multidisciplinary

Distance Determination in Proteins inside Xenopus laevis Oocytes by Double Electron-Electron Resonance Experiments

Ryuji Igarashi et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)

Article Multidisciplinary Sciences

Site-directed spin labeling of a genetically encoded unnatural amino acid

Mark R. Fleissner et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)

Article Chemistry, Medicinal

Large-scale synthesis of a persistent trityl radical for use in biomedical EPR applications and imaging

Ilirian Dhimitruka et al.

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS (2007)

Article Biochemistry & Molecular Biology

Reversible reduction of nitroxides to hydroxylamines: Roles for ascorbate and glutathione

Andrey A. Bobko et al.

FREE RADICAL BIOLOGY AND MEDICINE (2007)

Article Chemistry, Physical

Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance

Gunnar Jeschke et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2007)

Article Biochemistry & Molecular Biology

From the test tube to the cell: Exploring the floding and aggregation of a beta-clam protein

Zoya Ignatova et al.

BIOPOLYMERS (2007)

Article Radiology, Nuclear Medicine & Medical Imaging

A gadolinium triacetic monoamide DOTA derivative with a methanethiosulfonate anchor group. Relaxivity properties and conjug

David Thonon et al.

CONTRAST MEDIA & MOLECULAR IMAGING (2007)

Article Biochemistry & Molecular Biology

Reconstruction of the chemotaxis receptor-kinase assembly

SY Park et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2006)

Article Physics, Atomic, Molecular & Chemical

DeerAnalysis2006 - a comprehensive software package for analyzing pulsed ELDOR data

G. Jeschke et al.

APPLIED MAGNETIC RESONANCE (2006)

Review Biochemistry & Molecular Biology

Peroxiredoxins: A historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling

SG Rhee et al.

FREE RADICAL BIOLOGY AND MEDICINE (2005)

Article Chemistry, Physical

Electron dipole-dipole interaction in ESEEM of nitroxide biradicals

LV Kulik et al.

CHEMICAL PHYSICS LETTERS (2001)

Article Chemistry, Physical

Dipolar spectroscopy and spin alignment in electron paramagnetic resonance

G Jeschke et al.

CHEMICAL PHYSICS LETTERS (2000)

Article Biochemical Research Methods

Dead-time free measurement of dipole-dipole interactions between electron spins

M Pannier et al.

JOURNAL OF MAGNETIC RESONANCE (2000)