期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 199, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2021.114036
关键词
Cissus quadrangularis L.; Phenolic compounds; Stilbenes; Dietary supplements; UHPLC-PDA-MS; LC-QToF; HPTLC
资金
- Center for Food Safety and Applied Nutrition, US Food and Drug Administration [5U01FD004246]
- United States Department of Agriculture, Agricultural Research Service [58-6060-6-015]
This study focused on the simultaneous determination of polyphenolic compounds in Cissus quadrangularis using UHPLC-PDA-MS, obtaining fingerprints of stem and leaf extracts and characterizing polyphenolics through LC-QToF analysis. Over 40 components were structurally correlated from LC-QToF spectra, and a simple, fast HPTLC method was developed for qualitative analysis and quality assessment of dietary supplements.
Stem and leaf of Cissus quadrangularis L. (Vitaceae), indigenous to Asia and Africa, were used for medicinal and dietary purposes with limited information about the plant's phytochemistry. Stem and leaf samples were assessed for the simultaneous determination of polyphenolic compounds (catechin, epicatechin, quercetin-3-O-beta-glucopyranoside, kaempferol-3-O-beta-glucoside, quercetin-3-O-beta-rhamnoside, leachianol F, amurensin A, pallidol, resveratrol, and quadrangularin A), using UHPLC-PDA-MS. The validation data showed that the method is precise, specific, accurate, and linear over the range of 0.5-100 mu g/mL. Reversed-phase ultra-high performance liquid chromatography (UHPLC) fingerprints of the crude methanolic stem and leaf extracts of C. quadrangularis were obtained at different wavelengths based on their lambda(max). Polyphenolics were characterized using both UHPLC-PDA-MS and LC-QToF analysis. From liquid chromatography quadrupole time of flight-electrospray ionization mass spectrometry (LC-QToF) spectra, over 40 components were structurally correlated, and confirmation was based on the fragmentation characteristics and also from the information available in the literature. In addition to the LC-QToF method, a simple, fast HPTLC method was developed as a visual aid for the rapid qualitative analytical tool to help establish the quality assessment of botanical raw materials and dietary supplements claiming to contain Cissus. (C) 2021 Elsevier B.V. All rights reserved.
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