Metal organic framework-801 based magnetic solid-phase extraction and its application in analysis of preterm labor treatment drugs

A novel magnetic solid-phase extraction (MSPE) method based on metal organic framework-801 (MOF-801) modified magnetic nanoparticles (noted as PEI-MNPs@MOF-801) was successfully prepared for the extraction of preterm labor treatment drugs (including indometacin, acemetacin and sulindac) from human plasma sample. MOF-801, a new kind of porous coordination polymer composed of Zr4+ and fumaric acid, was modified on the surface of synthesized polyethyleneimine magnetic nanoparticles (PEI-MNPs) through amidation reaction. The obtained PEI-MNPs@MOF-801 was characterized with Fourier-transformed infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction and transmission electron microscopy. A MSPE-HPLC-UV method was developed by coupling PEI-MNPs@MOF-801 with HPLC system. Several parameters that affect the extraction efficiency including acetonitrile content, NaCl content, extraction time and sample volume were investigated. Under optimum conditions, the proposed MSPE-HPLC-UV method showed high extraction efficiency (enrichment factors between 96-118), good linearity with R >= 0.9987, excellent reproducibility (RSD <= 4.30 %) and low limits of detection in the range of 0.03-0.05 ng/mL. This method was also successfully applied to the extraction of indometacin, acemetacin and sulindac in human plasma samples and good recoveries were obtained. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

A novel magnetic solid-phase extraction method based on MOF-801 modified magnetic nanoparticles was developed for the extraction of preterm labor treatment drugs from human plasma, showing high extraction efficiency and low detection limits.