Multiple-fingerprint analysis of Poria cocos polysaccharide by HPLC combined with chemometrics methods

In this study, the multiple fingerprints, which were integrated with HPGFC-ELSD (high performance gel filtration chromatography - evaporative light scattering detector) fingerprint, PMP-HPLC-DAD (1-phenyl-3-methyl-5-pyrazolone-high performance liquid chromatography - diode array detector) fingerprint of complete acid hydrolysates and HILIC-HPLC-ELSD (hydrophilic interaction - high performance liquid chromatography - evaporative light scattering detector) fingerprint of enzyme hydrolysates, were established to evaluate the quality of polysaccharides from Poria cocos (PCPs). The similarity evaluation showed that 16 batches of PCPs from different origins had high similarity in structural characteristics based on the multiple fingerprints. The chromatographic data of multiple fingerprints of PCPs were fused, processed and analyzed by chemometric methods including HCA (hierarchical cluster analysis), PCA (principal component analysis) and PLS-DA (partial least squares discriminant analysis). The 16 batches of PCPs were divided into 3 categories in PCA, indicating a certain relationship between the structural characteristics and the origins. PLS-DA analysis indicated that Man, Glc, Gal, Fuc, the components with m/z of 2.22 x 10(4)similar to 1.53 x 10(4) Da and 3.46 x 10(3)similar to 2.69 x 10(3) Da, oligosaccharides with DPs of 6 and 7, respectively, could be regarded as potential chemical markers for the classification of PCPs from different origins. According to the multiple fingerprints and chemometric analysis, the two commercial samples were proved to be adulterants. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

This study established multiple fingerprints to evaluate the quality of polysaccharides from Poria cocos (PCPs). Chemometric methods were used to analyze chromatographic data, distinguishing 16 batches of PCPs from different origins and identifying potential chemical markers for classification. The results revealed the presence of adulterants in two commercial samples.