4.6 Article

N-Acyl-Homoserine Lactones May Affect the Gut Health of Low-Birth-Weight Piglets by Altering Intestinal Epithelial Cell Barrier Function and Amino Acid Metabolism

期刊

JOURNAL OF NUTRITION
卷 151, 期 7, 页码 1736-1746

出版社

OXFORD UNIV PRESS
DOI: 10.1093/jn/nxab104

关键词

low birth weight; intestine; microbiota; quorum-sensing signaling molecule; piglet

资金

  1. National Nature Science Foundation of China [31902189, 31630074, 31972596, 32002189, 31902170]
  2. Beijing Municipal Natural Science Foundation [S170001]
  3. Fundamental Research Funds for the Central Universities [2662020DKQD004, 2020TC066]

向作者/读者索取更多资源

The study revealed that LBW piglets had lower microbial diversity in the intestines compared to NBW piglets, while exhibiting higher concentrations of 3OC12-HSL which was negatively correlated with specific microbial populations.
Background: In piglets, low birth weight (LBW) is associated with intestinal dysfunction, which affects their growth performance and causes economic losses. Objectives: This study was designed to test whether microbial quorum sensing (QS) affects LBW-induced intestinal developmental defects in piglets. Methods: Seven normal-birth-weight (NBW; 1.36 +/- 0.01 kg) and 7 LBW (0.89 +/- 0.01 kg) piglets were selected. Feces were collected from piglets on 2, 21, and 50 days of age for detection of the QS signaling molecules, N-acyl-homoserine lactones (AHLs), and microbiota analysis. The associations between 2 long-chain AHLs [N-3-oxo-dodecanoyl-L-homoserine lactone (3OC12-HSL) and N-3-oxo-tetradecanoyl-L-homoserine lactone (30C14-HSL)1 and the microbes were tested using Spearman correlation coefficients. The effect of 3OC12-HSL and 3OC14-HSL on intestinal porcine epithelial cell-jejunum 2 (IPEC-J2) cell viability was investigated by cholecystokinin octapeptide assay. Transcriptomic analysis was performed by RNA sequencing on cells treated with 3OC12-HSL. Results: The concentrations of 3OC12-HSL and 3OC14-HSL in the feces of LBW piglets were higher than those in NBW piglets at age 50 d by 2.5- and 2.24-fold, respectively (P < 0.05). The microbial (Y diversity (observed species, abundancebased coverage estimator, and Shannon index) of LBW piglets was 81-91% lower than that of NBW piglets (P < 0.05). The relative abundance of Ruminococcaceae UCG-002/UCG-013 was 43.0% and 30.0% lower, respectively, in feces from LBW compared with NBW piglets (P < 0.05). 3OC12-HSL and Ruminococcaceae UCG-002/UCG-005/UCG-010 abundance were negatively correlated (rho <= -0.58). Treatment with 400 mu M 3OC12-HSL markedly reduced IPEC-J2 cell viability by 47.5%. Transcriptomic data showed that 3OC12-HSL mainly changed the import across plasma membrane and arginine and proline metabolism of IPEC-J2 cells. Conclusions: 3OC12-HSL is a QS signaling molecule with an ability to impair gut health of LBW piglets. This finding adds to our understanding of the mechanisms responsible for gut injury in LBW piglets.

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