Temporal Relations between Cortical Network Oscillations and Breathing Frequency during REM Sleep

Nasal breathing generates a rhythmic signal which entrains cortical network oscillations in widespread brain regions on a cycle-to-cycle time scale. It is unknown, however, how respiration and neuronal network activity interact on a larger time scale: are breathing frequency and typical neuronal oscillation patterns correlated? Is there any directionality or temporal relationship? To address these questions, we recorded field potentials from the posterior parietal cortex of mice together with respiration during REM sleep. In this state, the parietal cortex exhibits prominent theta and gamma oscillations while behavioral activity is minimal, reducing confounding signals. We found that the instantaneous breathing frequency strongly correlates with the instantaneous frequency and amplitude of both theta and gamma oscillations. Cross-correlograms and Granger causality revealed specific directionalities for different rhythms: changes in theta activity precede and Granger-cause changes in breathing frequency, suggesting control by the functional state of the brain. On the other hand, the instantaneous breathing frequency Granger causes changes in gamma frequency, suggesting that gamma is influenced by a peripheral reafference signal. These findings show that changes in breathing frequency temporally relate to changes in different patterns of rhythmic brain activity. We hypothesize that such temporal relations are mediated by a common central drive likely to be located in the brainstem.

Automated summary

The study found a strong correlation between instantaneous breathing frequency and the frequency and amplitude of theta and gamma oscillations in the brain. Changes in theta activity were found to precede and cause changes in breathing frequency, indicating control by the brain's functional state. On the other hand, changes in breathing frequency were found to cause changes in gamma frequency, suggesting influence by a peripheral reafference signal.