期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 125, 期 6, 页码 2461-2479出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00452.2020
关键词
auditory; deconvolution; inner hair cell; spiral ganglion; synapses
资金
- National Institute on Deafness and Other Communication Disorders (NIDCD) [R01-009913, R01-DC-006476, F32-DC-013721]
- NIDCD [P30-DC-005211]
- David M. Rubenstein Fund for Hearing Research
- John Mitchell, Jr. Trust
- Oberndorf family
The study revealed that pillar synapses mature later than modiolar synapses in the hair cell-spiral ganglion neuron synapse, with a significant difference in release process which may partly drive afferent firing properties.
Spiral ganglion neurons (SGNs) form single synapses on inner hair cells (IHCs), transforming sound-induced IHC receptor potentials into trains of action potentials. SGN neurons are classified by spontaneous firing rates as well as their threshold response to sound intensity levels. We investigated the hypothesis that synaptic specializations underlie mouse SGN response properties and vary with pillar versus modiloar synapse location around the hair cell. Depolarizing hair cells with 40 mM K thorn increased the rate of postsynaptic responses. Pillar synapses matured later than modiolar synapses. Excitatory postsynaptic current (EPSC) amplitude, area, and number of underlying events per EPSC were similar between synapse locations at steady state. However, modiolar synapses produced larger monophasic EPSCs when EPSC rates were low and EPSCs became more multiphasic and smaller in amplitude when rates were higher, while pillar synapses produced more monophasic and larger EPSCs when the release rates were higher. We propose that pillar and modiolar synapses have different operating points. Our data provide insight into underlying mechanisms regulating EPSC generation. NEW & NOTEWORTHY Data presented here provide the first direct functional evidence of late synaptic maturation of the hair cell- spiral ganglion neuron synapse, where pillar synapses mature after postnatal day 20. Data identify a presynaptic difference in release during stimulation. This difference may in part drive afferent firing properties.
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