期刊
JOURNAL OF NEURAL ENGINEERING
卷 18, 期 5, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1741-2552/abf28f
关键词
NIR neurostimulation; ex-vivo peripheral nerves; recruitment curve; optical single shots; sensitization effect; stimulation threshold; high spatial resolution
资金
- Walloon Region, Belgium-Pole Mecatech [NEUROPV no7740]
The study successfully designed a nerve chamber allowing ex-vivo electrical and optical stimulation for exploring the effect of key parameters in peripheral nerves. It was found that adjusting the duration and power of light pulses can effectively stimulate nerves, and repeated stimuli can enhance neural conduction effects.
Objective. To design and implement a setup for ex-vivo optical stimulation for exploring the effect of several key parameters (optical power and pulse duration), activation features (threshold, spatial selectivity) and recovery characteristics (repeated stimuli) in peripheral nerves. Approach. A nerve chamber allowing ex-vivo electrical and optical stimulation was designed and built. A 1470 nm light source was chosen to stimulate the nerve. A photodiode module was implemented for synchronization of the electrical and optical channels. Main results. Compound neural action potentials (CNAPs) were successfully generated with infrared light pulses of 200-2000 mu s duration and power in the range of 3-10 W. These parameters determine a radiant exposure for stimulation in the range 1.59-4.78 J cm(-2). Recruitment curves were obtained by increasing durations at a constant power level. Neural activation threshold is reached at a mean radiant exposure of 3.16 +/- 0.68 J cm(-2) and mean pulse energy of 3.79 +/- 0.72 mJ. Repetition rates of 2-10 Hz have been explored. In eight out of ten sciatic nerves (SNs), repeated light stimuli induced a sensitization effect in that the CNAP amplitude progressively grows, representing an increasing number of recruited fibres. In two out of ten SNs, CNAPs were composed of a succession of peaks corresponding to different conduction velocities. Significance. The reported sensitization effect could shed light on the mechanism underlying infrared neurostimulation. Our results suggest that, in sharp contrast with electrical stimuli, optical pulses could recruit slow fibres early on. This more physiological order of recruitment opens the perspective for specific neuromodulation of fibre population who remained poorly accessible until now. Short high-power light pulses at wavelengths below 1.5 mu m offer interesting perspectives for neurostimulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据