Formulation, optimization, and characterization of whey protein isolate nanocrystals for celecoxib delivery

Aim Current study aimed to improve the solubility and release profile of the celecoxib for cancer application. However, the low water solubility of celecoxib limited its application for cancer chemotherapy. Hence, new drug delivery-based approaches are compulsory for the efficient delivery of hydrophobic celecoxib for chemotherapy. Methods The celecoxib-loaded nanocrystals were prepared by anti-solvent precipitation-ultrasonication technique, and the formulation was optimised through various process parameters. Results The optimised formulation had an average particle diameter of 171.09 +/- 6.23 nm, with a PDI of 0.123 +/- 0.009 and high ZP -27.3 +/- 0.2 mV. The optimised formulation was stable, had higher entrapment efficiency 97.26 +/- 1.12%. The conformational changes in the denatured protein solution were detected through fluorescence spectroscopy. The transmission electron microscopy investigation showed rod-shaped nanocrystals morphology, and no chemical interactions were observed in optimised formulation through FTIR. The DSC and PXRD analysis exhibited an amorphous state of the freeze-dried formulation drug. Also, optimised nanocrystals enhance drug solubility around 26.01-fold, 15.51-fold and 19.08-fold in purified water, pH 6.8 and pH 7.4, and accomplish sustained drug delivery, respectively. Conclusion It can be concluded that biopolymer-coated celecoxib nanocrystals might be potential drug delivery of hydrophobic molecules for cancer therapy.

Automated summary

The study aimed to improve the solubility and release profile of celecoxib for cancer application. The optimized formulation of celecoxib-loaded nanocrystals showed stable characteristics with improved drug solubility and sustained drug delivery, making it a potential drug delivery system for cancer therapy.