期刊
JOURNAL OF MEDICINAL CHEMISTRY
卷 64, 期 8, 页码 4396-4409出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jmedchem.1c00051
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资金
- LogicLab ITN - European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant [813920]
Multiple diseases are associated with altered endothelial function, and endothelial dysfunction contributes to their pathophysiology. Biochemical changes of the dysfunctional endothelium are linked to various cellular organelles, requiring organelle-specific insight for better understanding. Raman imaging, combining chemical specificity with microscopic resolution, is useful for detecting biochemical changes in ED at the cellular level.
Multiple diseases are at some point associated with altered endothelial function, and endothelial dysfunction (ED) contributes to their pathophysiology. Biochemical changes of the dysfunctional endothelium are linked to various cellular organelles, including the mitochondria, endoplasmic reticulum, and nucleus, so organelle-specific insight is needed for better understanding of endothelial pathobiology. Raman imaging, which combines chemical specificity with microscopic resolution, has proved to be useful in detecting biochemical changes in ED at the cellular level. However, the detection of spectroscopic markers associated with specific cell organelles, while desirable, cannot easily be achieved by Raman imaging without labeling. This critical review summarizes the current advances in Raman-based analysis of ED, with a focus on a new approach involving molecular Raman reporters that could facilitate the study of biochemical changes in cellular organelles. Finally, imaging techniques based on both conventional spontaneous Raman scattering and the emerging technique of stimulated Raman scattering are discussed.
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