Inhibition of perchlorate biodegradation by ferric and ferrous iron

Previous observations from in-situ biological treatments in the subsurface of a perchlorate-contaminated site revealed multiple reduction processes occurring parallel to perchlorate degradation. Iron reduction was accelerated and correlated with a decline in the efficiency of the in-situ perchlorate reduction. In the current study, we examined the influence of iron forms on perchlorate reduction. A series of kinetic laboratory experiments were conducted, using an indigenous mixed perchlorate-reducing culture, enriched from the polluted soil that was undergoing bioremediation. The results show that ferrous iron was a non-competitive inhibitor with a 41% decrease in mu(max) for perchlorate reduction. Moreover, chlorate was accumulated in all samples treated with ferrous iron, indicating a disruption to the chlorate reduction step. Ferric iron, however, had less impact on perchlorate degradation with non-competitive inhibition reaching a 23% decrease in mu(max). Scanning electron microscopy (SEM) revealed that the presence of ferrous iron in the perchlorate degradation enrichment culture initiated cell encrustation. We propose that during perchlorate reduction and the emission of oxygen from chlorite dismutation, the chemical oxidation of ferrous iron occurred near the bacteria's surface where the enzyme is located, forming an oxidized iron crust layer that can directly affect the perchlorate reduction enzymatic system.

Automated summary

The study revealed that ferrous iron has a significant impact on perchlorate reduction, leading to a decrease in the rate of perchlorate reduction and accumulation of chlorate in the samples. On the other hand, ferric iron had a smaller effect on perchlorate degradation. Scanning electron microscopy showed that the presence of ferrous iron initiated the formation of an oxidized iron crust layer on the bacteria's surface, potentially affecting the enzymatic system involved in perchlorate reduction.