Profiling microbial community structures and functions in bioremediation strategies for treating 1,4-dioxane-contaminated groundwater

Microbial community compositions and functional profiles were analyzed in microcosms established using aquifer materials from a former automobile factory site, where 1,4-dioxane was identified as the primary contaminant of concern. Propane or oxygen biostimulation resulted in limited 1,4-dioxane degradation, which was markedly enhanced with the addition of nutrients, resulting in abundant Mycobacterium and Methyloversatilis taxa and high expressions of propane monooxygenase gene, prmA. In bioaugmented treatments, Pseudonocardia dioxanivorans CB1190 or Rhodococcus ruber ENV425 strains dominated immediately after augmentation and degraded 1,4-dioxane rapidly which was consistent with increased representation of xenobiotic and lipid metabolism-related functions. Although the bioaugmented microbes decreased due to insufficient growth substrates and microbial competition, they did continue to degrade 1,4-dioxane, presumably by indigenous propanotrophic and heterotrophic bacteria, inducing similar community structures across bioaugmentation conditions. In various treatments, functional redundancy acted as buffer capacity to ensure a stable microbiome, drove the restoration of the structure and microbial functions to original levels, and induced the decoupling between basic metabolic functions and taxonomy. The results of this study provided valuable information for design and decision-making for ex-situ bioreactors and in-situ bioremediation applications. A metagenomicsbased understanding of the treatment process will enable efficient and accurate adjustments when encountering unexpected issues in bioremediation.

Automated summary

The study found that enhancing the degradation of 1,4-dioxane by adding nutrients resulted in increased expression of specific genes, leading to the degradation of organic pollutants and affecting the structure and function of microbial communities. Although the bioaugmented microbes decreased due to insufficient growth substrates and microbial competition, they continued to degrade 1,4-dioxane through indigenous propanotrophic and heterotrophic bacteria. Functional redundancy acted as a buffer capacity to ensure a stable microbiome and drove the restoration of microbial functions to original levels.