Analysis of accumulation and phytotoxicity mechanism of uranium and cadmium in two sweet potato cultivars

The purpose of this study was to reveal the accumulation and phytotoxicity mechanism of sweet potato (Ipomoea batatas L.) roots following exposure to toxic levels of uranium (U) and cadmium (Cd). We selected two accumulation-type sweet potato cultivars as experimental material. The varietal differences in U and Cd accumulation and physiological metabolism were analyzed by a hydroponic experiment. High concentrations of U and Cd inhibited the growth and development of sweet potato and damaged the microstructure of root. The roots were the main accumulating organs of U and Cd in both sweet potato. Root cell walls and vacuoles (soluble components) were the main distribution sites of U and Cd. The chemical forms of U in the two sweet potato varieties were insoluble and oxalate compounds, while Cd mainly combined with pectin and protein. U and Cd changed the normal mineral nutrition metabolism in the roots, and also significantly inhibited the photosynthetic metabolism of sweet potatoes. RNA-seq showed that the cell wall and plant hormone signal transduction pathways responded to either U or Cd toxicity in both varieties. The inorganic ion transporter and organic compound transporter in roots of both sweet potato varieties are sensitive to U and Cd toxicity.

Automated summary

This study aimed to uncover the accumulation and phytotoxicity mechanism of sweet potato roots after exposure to toxic levels of uranium and cadmium. Different sweet potato cultivars showed varietal differences in U and Cd accumulation, with high concentrations inhibiting growth and damaging root microstructure. U and Cd mainly accumulated in root cell walls and vacuoles, affecting mineral nutrition and photosynthetic metabolism, while RNA-seq analysis revealed responses in cell wall and plant hormone signal transduction pathways to U and Cd toxicity.