期刊
JOURNAL OF FISH DISEASES
卷 44, 期 8, 页码 1147-1153出版社
WILEY
DOI: 10.1111/jfd.13375
关键词
diagnostic sensitivity; diagnostic specificity; immunohistochemistry; polymerase chain reaction; prevalence; proliferative kidney disease
资金
- project PROFISH [CZ.02.1.01/0.0/0.0/16_019/0000869]
- European Regional Development Fund in the operational programme VVV MSMT
The study compared the sensitivity of different diagnostic methods for detecting the causative agent of proliferative kidney disease in salmonid fish. PCR methods showed higher detection rates compared to IHC, with qPCR32 having the best match with IHC. While both PCR methods had similar sensitivity, their specificity differed significantly.
Diagnostic accuracy of pathogen detection depends upon the selection of suitable tests. Problems can arise when the selected diagnostic test gives false-positive or false-negative results, which can affect control measures, with consequences for the population health. The aim of this study was to compare sensitivity of different diagnostic methods IHC, PCR and qPCR detecting Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonid fish and as a consequence differences in disease prevalence. We analysed tissue from 388 salmonid specimens sampled from a recirculating system and rivers in the Czech Republic. Overall prevalence of T. bryosalmonae was extremely high at 92.0%, based on positive results of at least one of the above-mentioned screening methods. IHC resulted in a much lower detection rate (30.2%) than both PCR methods (qPCR32: 65.4%, PCR: 81.9%). While qPCR32 produced a good match with IHC (60.8%), all other methods differed significantly (p < .001) in the proportion of samples determined positive. Both PCR methods showed similar sensitivity, though specificity (i.e., the proportion of non-diseased fish classified correctly) differed significantly (p < .05). Sample preservation method significantly (p < .05) influenced the results of PCR, with a much lower DNA yield extracted from paraffin-embedded samples. Use of different methods that differ in diagnostic sensitivity and specificity resulted in random and systematic diagnosis errors, illustrating the importance of interpreting the results of each method carefully.
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