期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 224, 期 12, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jeb.242205
关键词
Bafilomycin; Bufalin; Ion transporters; V-ATPase; Ionoregulation
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2015-05359]
The nuchal organ of Daphnia magna is a site for Na+ influx and H+, NH4+, and Cl- efflux. The study utilized scanning ion-selective electrode technique and inhibitors of specific transporters to investigate Na+ transport mechanisms across the nuchal organ, suggesting the involvement of Na+/H+ and Na+/NH4+ exchangers in the process.
The nuchal organ of the embryos and neonates of the cladoceran, Daphnia magna, has been shown to be a site of Na+ influx and H+, NH4+ and Cl- efflux. This study combines the scanning ion-selective electrode technique with application of inhibitors of specific transporters to assess the mechanisms of Na+ transport across the nuchal organ. Na+ influx across the nuchal organwas inhibited both by inhibitors of the Na+/K+-ATPase (ouabain, bufalin) and by inhibitors of the vacuolar H+-ATPase (bafilomycin, N-ethylmaleimde, 7-chloro-4nitrobenzo-2-oxa-1,3-diazole, KM91104, S-nitrosoglutathione). Na+ influx was unaffected by the epithelial Na+ channel blocker benzamil, but was sensitive to ethylisopropyl amiloride and elevated external ammonium concentrations, consistent with roles for Na+/H+ and Na+/NH4+ exchangers in the apical membrane but not Na+ channels. Transport across the basolateral membrane into the haemolymph is proposed to involve the Na+/K+-ATPase and a thiazide-sensitive Na+/Cl- cotransporter.
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