Female Marking via Rubidium-Labeled Ejaculates in the West Indian Sweetpotato Weevil (Coleoptera: Curculionidae)

The West Indian sweetpotato weevil, Euscepes postfasciatus (Fairmaire), is one of the pests of the sweet potato, Ipomoea batatas, in tropical and subtropical countries. Although an eradication program using the sterile insect technique (SIT) for this weevil is now underway in Japan, the lack of potent attractants such as sex pheromones for this weevil species prevents effective monitoring and hampers pest control operations. New methods to monitor the performance of sterile males using trapped wild females are needed. In this study, we tested whether the ejaculate of the labeled males that were fed an artificial diet labeled with the trace element, rubidium (Rb), is detectable in the fertilized females. We fed an artificial diet treated with Rb (1.00, 0.50, 0.25, and 0.00%) to adult male weevils for 14 d after emerging and quantified the rubidium content in inseminated females. We also examined the side effects of the artificial diet on mating performance and longevity. The advantages of monitoring the spatial level of sterility using data from females labeled with ejaculate of Rb-fed males in an SIT eradication program are discussed.

Automated summary

The West Indian sweetpotato weevil is a pest of sweet potatoes in tropical and subtropical countries. An eradication program using SIT is being conducted in Japan, but the lack of potent attractants like sex pheromones hampers pest control operations. This study tested whether the ejaculate of males fed with Rb can be detected in fertilized females and discussed the advantages of monitoring sterility levels in an SIT eradication program.