Methodology for characterization of platinum-based drug's targeted delivery nanosystems

Conventional anticancer therapies exploiting platinum-based drugs rely principally on the intravascular injection of the therapeutic agent. The anticancer drug is distributed throughout the body by the systemic blood circulation undergoing cellular uptake, rapid clearance and excretion. Consequently, only a small portion of the platinum-based drug reaches the tumor site, which is associated with severe side effects. For this reason, targeted delivery systems are of great need since they offer enhanced and selective delivery of a drug to cancerous cells making the therapy safe and more effective. Up to date, a variety of the Pt-based drug targeted delivery systems (Pt-based DTDSs) utilizing nanomaterials have been developed and tested using a range of analytical techniques that provided essential information on their synthesis, stability, biodistribution and cytotoxicity. Here we summarize those experimental techniques indicating their applicability at different stages of the research, as well as pointing out their strengths, advantages, drawbacks and limitations. Also, the existing strategies and approaches are critically reviewed with the objective to reveal and give rise to the development of the analytical methodology suitable for reliable Pt-based DTDSs characterization which would eventually result in novel therapies and better patients' outcomes.

Automated summary

Conventional platinum-based anticancer therapies face challenges in drug distribution and severe side effects, highlighting the need for targeted delivery systems. Utilizing nanomaterials to develop Pt-based drug targeted delivery systems offers enhanced and selective delivery to cancer cells, paving the way for safer and more effective treatments. Experimental techniques and analytical methodologies are crucial for the characterization and development of novel therapies utilizing Pt-based DTDSs.