Model-based evidence for the relevance of microbial community variability to the efficiency of the anaerobic reductive dechlorination of TCE

The composition of mixed dechlorinating communities varies considerably in field and laboratory conditions. Dechlorinators thrive alongside with distinctive populations that help or hinder dechlorination. The variability of the composition of dechlorinating communities inevitably precludes a firm consensus regarding the optimal strategies for biostimulation. This lack of consensus motivated a model-based approach for the investigation of how the variability of the composition of a microbial community impacts the electron donor supply strategies for accelerating chloroethene removal. To this end, a kinetic model accounting for dechlorination in conjunction with cooperative and competing processes was developed. Model parameters were estimated using a multiexperiment, multi-start algorithm and data from research previously performed with two generations of a methane-producing, Dehalococcoides mccartyi-dominated consortium. The two generations of the consortium functioned comparably under maintenance conditions but performed divergently under high electron donor surpluses. The multi-experiment, multi-start algorithm overcame the hurdles of poor parameter identifiability and offered a probable cause for the different behaviors exhibited by each of the two generations of the chloroethene-degrading consortium: modest differences in the make-up of non-dechlorinators, which were minority populations, significantly influenced the fate of the offered electron donor.

Automated summary

The composition of mixed dechlorinating communities varies significantly, affecting the rate of dechlorination. A model-based approach was used to investigate how the variability of microbial community composition impacts electron donor supply strategies. Differences in the makeup of non-dechlorinators may have a significant influence on the utilization of the offered electron donor.