期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 59, 期 6, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02959-20
关键词
artificial intelligence; deep learning; RT-PCR; COVID-19; TaqPath; SARS-CoV-2; real-time PCR
类别
This study introduces a novel deep learning approach to interpret RT-PCR data, with the developed model serving as a quality assurance tool for real-time monitoring of result interpretation, applicable to pathogen detection such as SARS-CoV-2.
Real-time PCR (RT-PCR) is widely used to diagnose human pathogens. RT-PCR data are traditionally analyzed by estimating the threshold cycle (C-T) at which the fluorescence signal produced by emission of a probe crosses a baseline level. Current models used to estimate the C-T value are based on approximations that do not adequately account for the stochastic variations of the fluorescence signal that is detected during RT-PCR. Less common deviations become more apparent as the sample size increases, as is the case in the current SARS-CoV-2 pandemic. In this work, we employ a method independent of C-T value to interpret RT-PCR data. In this novel approach, we built and trained a deep learning model, qPCRdeepNet, to analyze the fluorescent readings obtained during RT-PCR. We describe how this model can be deployed as a quality assurance tool to monitor result interpretation in real time. The model's performance with the TaqPath COVID19 Combo Kit assay, widely used for SARS-CoV-2 detection, is described. This model can be applied broadly for the primary interpretation of RT-PCR assays and potentially replace the C-T interpretive paradigm.
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