期刊
JOURNAL OF CLINICAL LABORATORY ANALYSIS
卷 35, 期 6, 页码 -出版社
WILEY
DOI: 10.1002/jcla.23783
关键词
auditory neuropathy; cochlea; CYLD; hearing; neurite
资金
- National Key R&D Program of China [2018YFA0107001]
- National Natural Science Foundation of China [31970749, 32070787]
By studying Cyld KO mice and nerve cell lines, it was found that CYLD protein might be associated with auditory neuropathy, and knocking down CYLD significantly reduces the length and proportion of neurites growing from nerve cells.
Background Auditory neuropathy is a cause of hearing loss that has been studied in a number of animal models. Signal transmission from hair cells to spiral ganglion neurons plays an important role in normal hearing. CYLD is a microtubule-binding protein, and deubiquitinase involved in the regulation of various cellular processes. In this study, we used Cyld knockout (KO) mice and nerve cell lines to examine whether CYLD is associated with auditory neuropathy. Methods Hearing of Cyld KO mice was studied using the TDT RZ6 auditory physiology workstation. The expression and localization of CYLD in mouse cochlea and cell lines were examined by RT-PCR, immunoblotting, and immunofluorescence. CYLD expression was knocked down in SH-SY5Y cells by shRNAs and in PC12 and N2A cells by siRNAs. Nerve growth factor and retinoic acid were used to induce neurite outgrowth, and the occurrence and length of neurites were statistically analyzed between knockdown and control groups. Results Cyld KO mice had mild hearing impairment. Moreover, CYLD was widely expressed in mouse cochlear tissues and different nerve cell lines. Knocking down CYLD significantly reduced the length and proportion of neurites growing from nerve cells. Conclusions The abnormal hearing of Cyld KO mice might be caused by a decrease in the length and number of neurites growing from auditory nerve cells in the cochlea, suggesting that CYLD is a key protein affecting hearing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据