期刊
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
卷 96, 期 9, 页码 2596-2603出版社
WILEY
DOI: 10.1002/jctb.6802
关键词
polyhydroxyalkanoates; microwave-assisted extraction; mixed microbial cultures; bacterial biopolymer; biopolymer extraction
类别
资金
- project ERANET/LAC [ELAC2015/T03-0715-ValBio-3D]
- project DIUFRO [DI19-1003]
MAE is an effective method for recovering PHAs from mixed microbial cultures with high extraction efficiency and quality, similar to conventional heat extraction. Optimal conditions for MAE include a 1-minute extraction at 130 degrees Celsius with a chloroform-to-methanol ratio of 1:1 and a solvent-to-biomass ratio of 20 volumes.
BACKGROUND: Polyhydroxyalkanoates (PHAs) are a class of biodegradable biopolymers produced and accumulated by microorganisms. PHAs are recovered from microbial biomass through the stages of pretreatment, extraction and purification. An alternative recovery method is microwave-assisted extraction (MAE), which involves the supply of energy through an electromagnetic field, generating high temperatures in reduced times. A study to evaluate the recovery of PHAs from mixed microbial cultures was conducted using MAE. This method was evaluated through a four-factorial experimental design, with the efficiency as the evaluated response. PHAs were also recovered using conventional heat extraction as control. RESULTS: It was found that the highest efficiency achieved using MAE was 68 +/- 1.2% (w/w), which was close to the 66 +/- 5.3% (w/w) obtained using conventional heat extraction. The following combination of factors corresponded to the optimum conditions of experimental design: extraction time of 1 min, temperature of 130 degrees C, chloroform-to-methanol ratio of 1:1 and solvent-to-biomass ratio of 20 volumes. The efficiency increased substantially when the solvent-to-biomass ratio and chloroform-to-methanol ratio increased, although the temperature was the least significant factor. Functional groups, decomposition temperatures (263-309 degrees C) and melting point (158 degrees C) of MAE-recovered PHAs were similar to those measured for commercial poly[(3-hydroxybutyrate)-co-(3-hydroxyvalerate)]. CONCLUSIONS: MAE is a never-before-reported useful method for recovering PHAs from mixed microbial cultures, which provides for a high extraction efficiency and quality of the recovered biopolymers. (c) 2021 Society of Chemical Industry (SCI).
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