期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 236, 期 11, 页码 7450-7463出版社
WILEY
DOI: 10.1002/jcp.30417
关键词
cellular elasticity; electrical stimulation; F‐ actin; gelsolin; intracellular Ca2+
资金
- Creative Industry Professional Development Program through the Ministry of Trade and Industry Energy [20002725]
- National Research Foundation of Korea [2020R1F1A1057505]
- Kyung Hee University [KHU-20210133]
- National Research Foundation of Korea [2020R1F1A1057505] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Cellular elasticity is a key factor in physiological and pathological processes, serving as a potential biomarker for cellular state. Both internal and external stimuli can affect cellular elasticity, leading to changes in cellular characteristics or function. The application of electric fields is a promising method to alter cellular elasticity, with mechanisms underlying its effect remaining unknown.
Cellular elasticity is a key factor related to a broad range of physiological and pathological processes. The elasticity of a single cell has thus emerged as a potential biomarker to characterize the cellular state. Both internal and external stimuli affect cellular elasticity, and changes in elasticity can cause alterations in cellular characteristics or function. The application of electric fields (EFs) is a promising method that can be used to change cellular elasticity; however, the mechanisms underlying its effect remain unknown. Here, we demonstrate EFs-induced elasticity changes in human dermal fibroblasts and discuss the underlying mechanism related to actin polymerization. Cellular elasticity increases after EF (50 mV/mm) stimulation, reaching a maximum at 30 min before decreasing between 30 and 120 min. The cellular elasticity under EF stimulation, regardless of stimulation time, is higher than that of the control. F-actin regulates the elasticity of cells through gelsolin activation. We show changes in intracellular Ca2+ caused by EFs, which induced gelsolin activation and F-actin content changes. This result demonstrates a series of processes in which external electrical stimulation conditions regulate cellular elasticity.
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