期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 25, 期 10, 页码 4870-4876出版社
WILEY
DOI: 10.1111/jcmm.16465
关键词
apoptosis; CD8(+) T cells; homeostasis; IL‐ 7R signalling; Med1
资金
- Major International (Regional) Joint Research Project [81820108017]
- Natural Science Foundation of China [81771673]
- Young Talent Program of Xi'an Jiaotong University [YX1J005]
- COVID-19 special project of Xi'an Jiaotong University Foundation [xzy032020002]
- COVID-19 special project - Qinnong Bank and Xi'an Jiaotong University [QNXJTU-01]
- Natural Science Foundation of Shaanxi Province [2020JM-065, 2020JQ-098]
- China Postdoctoral Science Foundation [2019M653673]
Med1 plays an important role in maintaining CD8(+) T cells through IL-7R alpha/STAT5 pathway-mediated cell survival. Med1 deficiency leads to increased cell death, decreased expression of IL-7R alpha, and down-regulation of pSTAT5 in CD8(+) T cells.
Under steady-state conditions, the pool size of peripheral CD8(+) T cells is maintained through turnover and survival. Beyond TCR and IL-7R signals, the underlying mechanisms are less well understood. In the present study, we found a significant reduction of CD8(+) T cell proportion in spleens but not in thymi of mice with T cell-specific deletion of Mediator Subunit 1 (Med1). A competitive transfer of wild-type (WT) and Med1-deficient CD8(+) T cells reproduced the phenotype in the same recipients and confirmed intrinsic role of Med1. Furthermore, we observed a comparable degree of migration and proliferation but a significant increase of cell death in Med1-deficient CD8(+) T cells compared with WT counterparts. Finally, Med1-deficient CD8(+) T cells exhibited a decreased expression of interleukin-7 receptor alpha (IL-7R alpha), down-regulation of phosphorylated-STAT5 (pSTAT5) and Bim up-regulation. Collectively, our study reveals a novel role of Med1 in the maintenance of CD8(+) T cells through IL-7R alpha/STAT5 pathway-mediated cell survival.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据