期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 25, 期 12, 页码 5391-5403出版社
WILEY
DOI: 10.1111/jcmm.16550
关键词
human umbilical vein endothelial cells; long non‐ coding RNA ATB; MiR‐ 651‐ 3p; preeclampsia; spiral artery remodelling; trophoblasts; YY1
资金
- National Natural Science Foundation of China [81571465, 81871175]
The study showed that lncRNA-ATB was down-regulated in the placenta of preeclampsia patients, and knockdown of lncRNA-ATB decreased migration and invasion of trophoblast cells. MiR-651-3p was a direct target of lncRNA-ATB with opposite effects, and it targeted YY1 to affect its expression and inhibitory effects on biological functions.
Our previous studies have confirmed that lncRNA-ATB may be involved in the pathogenesis of preeclampsia, however, it is uncertain whether lncRNA-ATB influence the interaction between trophoblast and endothelial cells, which is crucial to the uterine spiral artery remodelling. Scratch wound healing and transwell invasion assay were conducted to test the migration and invasion of trophoblast cells. Co-culture model was used to simulate the physiological environment in vivo. The expression levels of lncRNA-ATB were analyzed in placenta tissues from healthy pregnant women and preeclampsia patients. Subsequently, the binding site of lncRNA-ATB and miR-651-3p was verified using dual-luciferase reporter assay, and the rescue experiment was used to study the effects of these two on the biological function. The direct effects of miR-651-3p and Yin Yang 1 (YY1) were verified using similar methods. LncRNA-ATB was found to be down-regulated in the placenta of preeclampsia patients. LncRNA-ATB knockdown decreased trophoblast migration, invasion and colocalisation with human umbilical vein endothelial cells. MiR-651-3p was a direct target of lncRNA-ATB and they had opposite effects. Moreover, the expression of lncRNA-ATB and miR-651-3p in placental tissues was negatively correlated. MiR-651-3p has been confirmed to directly target the 3 ' untranslated region of YY1. The inhibitory effects of YY1 low expression on biological function was rescued by miR-651-3p depletion. Western blot analysis showed that lncRNA-ATB could regulate YY1 expression by sponging miR-651-3p. LncRNA-ATB functioned as a competitive endogenous RNA of miR-651-3p to regulate YY1 on progress of spiral artery remodelling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据