期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 25, 期 11, 页码 5335-5338出版社
WILEY
DOI: 10.1111/jcmm.16488
关键词
apoptosis; celastrol; cervical cancer; mitochondrial fragmentation; proliferation
The study demonstrated that celastrol has anticancer potential against HeLa cervical cancer cells by down-regulating MMP-2 and MMP-9 expression, promoting apoptosis, and inducing mitochondrial fragmentation.
The present study evaluated the anticancer potential of celastrol through down-regulation of matrix metalloproteinase-2 (MMP-2) and MMP-9. HeLa cells were incubated with different concentrations of celastrol (1, 10 and 100 mu M) for 48h. Doxorubicin was used as a reference drug. Cancer cell migration, apoptosis, cell viability and mitochondrial fragmentation were evaluated following celastrol treatment. In addition, the expression level of MMP-2, MMP-9 and caspase-3 was evaluated following celastrol treatment. HeLa cell viability was 94.1 +/- 7, 53.4 +/- 4 and 36.3 +/- 2% at 1-100 mu M of celastrol, respectively. Apoptotic cell numbers were increased, and inhibition of larger wounds in cancer cells was observed following celastrol treatment. Celastrol-treated cells showed condensed nuclei and clumped mitochondria. Reduced expression of MMP-2 and MMP-9 and increased expression of caspase-3 were observed following celastrol treatment. Based on the experimental results, we are concluding that the celastrol was effective against HeLa cervical cancer cells.
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