期刊
JOURNAL OF CELL SCIENCE
卷 134, 期 10, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.248559
关键词
Bulk moduli; Heterogeneity; Lamin A/C; Mechanotransduction
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN/05843-2014, EQPEQ/472339-2015, RTI/00348-2018]
- Canadian Institutes of Health Research (CIHR) [143327]
- Canadian Foundation for Innovation [32749]
- McGill University
The study found that nuclear stiffness is crucial for cellular response to stresses, with lamin A/C possibly playing a role, and nuclear compression is anisotropic. Additionally, the distribution of lamin A/C in the nuclear membrane may impact local nuclear deformations.
While diverse cellular components have been identified as mechanotransduction elements, the deformation of the nucleus itself is a critical mechanosensory mechanism, implying that nuclear stiffness is essential in determining responses to intracellular and extracellular stresses. Although the nuclear membrane protein lamin A/C is known to contribute to nuclear stiffness, bulk moduli of nuclei have not been reported for various levels of lamin A/C. Here, we measure the nuclear bulk moduli as a function of lamin A/C expression and applied osmotic stress, revealing a linear dependence within the range of 2-4 MPa. We also find that the nuclear compression is anisotropic, with the vertical axis of the nucleus being more compliant than the minor and major axes in the substrate plane. We then related the spatial distribution of lamin A/C with submicron 3D nuclear envelope deformation, revealing that local areas of the nuclear envelope with higher density of lamin NC have correspondingly lower local deformations. These findings describe the complex dispersion of nuclear deformations as a function of lamin A/C expression and distribution, implicating a lamin A/C role in mechanotransduction. This article has an associated First Person interview with the first author of the paper.
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