期刊
JOURNAL OF BIOPHOTONICS
卷 14, 期 10, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.202000488
关键词
hollow-core fibre; Raman fibre probe; Raman spectroscopy; SERDS
资金
- Cancer Research UK [A24867]
- Engineering and Physical Sciences Research Council [EP/S001123/1]
- Medical Research Council [MR/R017794/1]
- EPSRC [EP/S001123/1] Funding Source: UKRI
A portable, background and fluorescence free endoscopic Raman probe was developed using a unique optical fibre technology, allowing compatibility with standard bronchoscopes. By utilizing shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser, the probe successfully separated fluorescence and Raman spectrum from highly fluorescent samples, demonstrating its suitability for biomedical applications.
Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.
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