期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 296, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbc.2021.100664
关键词
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资金
- Fondazione Mariani
- Banca Intesa-San Paolo
- Fondazione Regionale per la Ricerca Biomedica [Care4NeuroRare CP_20/2018]
Nonphosphorylated tau protein can inhibit the elongation of Aβ(1-42) fibrils and structurally affect the aggregation process of Aβ. In in vivo experiments, tau monomers bind to Aβ(1-42) oligomers, hinder their interaction with anti-Aβ antibodies, and provide neuroprotective effects by shielding the hydrophobic patches on Aβ(1-42) oligomers.
The formation of neurofibrillary tangles and amyloid plaques accompanies the progression of Alzheimer's disease. Tangles are made of fibrillar aggregates formed by the microtubule-associated protein tau, whereas plaques comprise fibrillar forms of amyloid-beta (A beta). Both form toxic oligomers during aggregation and are thought to interact synergistically to each promote the accumulation of the other. Recent in vitro studies have suggested that the monomeric nonphosphorylated full-length tau protein hinders the aggregation of A beta(1-40) peptide, but whether the same is true for the more aggregationprone A beta(1-42) was not determined. We used in vitro and in vivo techniques to explore this question. We have monitored the aggregation kinetics of A beta(1-42) by thioflavine T fluorescence in the presence or the absence of different concentrations of nonphosphorylated tau. We observed that elongation of A beta(1-42) fibrils was inhibited by tau in a dose-dependent manner. Interestingly, the fibrils were structurally different in the presence of tau but did not incorporate tau. Surface plasmon resonance indicated that tau monomers bound to A beta(1-42) oligomers (but not monomers) and hindered their interaction with the anti-A beta antibody 4G8, suggesting that tau binds to the hydrophobic central core of A beta recognized by 4G8. Tau monomers also antagonized the toxic effects of A beta oligomers in Caenorhabditis elegans. This suggests that nonphosphorylated tau might have a neuroprotective effect by binding A beta(1-42) oligomers formed during the aggregation and shielding their hydrophobic patches.
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