Neochloris oleoabundans oil production in an outdoor tubular photobioreactor at pilot scale

Oil production was tested with Neochloris oleoabundans in a 6 m(3), horizontal soft sleeve tubular reactor from 22 October to 7 November in Matalascanas, southern Spain. Biomass productivity during the nitrogen replete phase was 7.4 g dw m(-2) day(-1). Maximum lipid content in the biomass was 39% and average lipid productivity during the nitrogen depletion phase was 2.0 g m(-2) day(-1). Nitrogen depletion of the cultures was carried out in order to enhance fatty acid formation, using the inverse nitrogen quota in the biomass to predict the fatty acid content. TFA concentration at harvest was 14% DW, compared to a value of 17%, predicted by the inverse nitrogen quota. The overall feasibility of the horizontal tubular technology for microalgal oil production, including mixing energy expenditure, was evaluated.

Automated summary

In this study, oil production using Neochloris oleoabundans was tested in a horizontal soft sleeve tubular reactor in southern Spain. The biomass productivity was higher during the nitrogen replete phase, while the lipid productivity remained stable during the nitrogen depletion phase. Additionally, nitrogen depletion was used to enhance fatty acid formation, with the inverse nitrogen quota in the biomass predicting the fatty acid content.