4.6 Article

Analysis of Cryptosporidium spp. from clinical samples by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

期刊

JOURNAL OF APPLIED MICROBIOLOGY
卷 131, 期 4, 页码 1840-1847

出版社

WILEY
DOI: 10.1111/jam.15077

关键词

Cryptosporidium; MALDI-TOF; mass spectrometry; matrix-assisted laser desorption/ionization time-of-flight; purification

资金

  1. Society for Applied Microbiology

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The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
Aim: To purify Cryptosporidium spp. oocysts from clinical stool samples and evaluate using an up-to-date mass spectrometry protocol producing high-quality reference spectra. Methods and Results: A refined purification protocol was developed for oocysts from stools, involving salt flotation and potassium bromide density centrifugation. Purified oocysts were prepared for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) by formic acid extraction, and the extracts analysed using the Bruker MALDI Biotyper system. Individual spectral markers were identified by their specific mass peaks. Cryptosporidium parvum oocysts (Iowa strain) propagated in vivo, and C. parvum (n = 2) and Cryptosporidium hominis (n = 1) oocysts from clinical stool samples produced distinct spectra that were considered specific to Cryptosporidium spp. with no evidence of contamination. Conclusions: The production of distinct spectra demonstrated the utility of the purification method for oocysts from clinical stool samples and provided reference spectra. Significance and Impact of the Study: The use of MALDI-TOF MS and other mass spectrometry techniques has been limited previously to C. parvum oocysts propagated in vivo. Appropriate purification of oocysts can achieve sufficient biomass, enabling analysis by MALDI-TOF MS and potentially other mass spectrometry platforms, facilitating peptide and protein discovery and identification of biomarkers from a much wider range of Cryptosporidium spp. from natural infections.

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