期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/ijms22073369
关键词
RBC storage lesion; RBC membrane proteome; RBC shape modifications; extracellular vesicles proteome; beta thalassemia trait donors; donor variation effect; network analysis
资金
- Hellenic Foundation for Research and Innovation (HFRI)
- General Secretariat for Research and Technology (GSRT) [2032]
Studying the effects of storage on red blood cells from donors with beta-thalassemia traits, researchers found that these cells cope better under stress and exhibit unique membrane proteome characteristics, including upregulation of certain proteins and downregulation of nitrogen-related transporters. These findings shed light on the physiology and aging of stored beta-thalassemia red blood cells.
Genetic characteristics of blood donors may impact the storability of blood products. Despite higher basal stress, red blood cells (RBCs) from eligible donors that are heterozygous for beta-thalassemia traits (beta Thal(+)) possess a differential nitrogen-related metabolism, and cope better with storage stress compared to the control. Nevertheless, not much is known about how storage impacts the proteome of membrane and extracellular vesicles (EVs) in beta Thal(+). For this purpose, RBC units from twelve beta Thal(+) donors were studied through proteomics, immunoblotting, electron microscopy, and functional ELISA assays, versus units from sex- and aged-matched controls. beta Thal(+) RBCs exhibited less irreversible shape modifications. Their membrane proteome was characterized by different levels of structural, lipid raft, transport, chaperoning, redox, and enzyme components. The most prominent findings include the upregulation of myosin proteoforms, arginase-1, heat shock proteins, and protein kinases, but the downregulation of nitrogen-related transporters. The unique membrane proteome was also mirrored, in part, to that of beta Thal(+) EVs. Network analysis revealed interesting connections of membrane vesiculation with storage and stress hemolysis, along with proteome control modulators of the RBC membrane. Our findings, which are in line with the mild but consistent oxidative stress these cells experience in vivo, provide insight into the physiology and aging of stored beta Thal(+) RBCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据