4.7 Article

BiFC Method Based on Intraorganellar Protein Crowding Detects Oleate-Dependent Peroxisomal Targeting of Pichia pastoris Malate Dehydrogenase

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出版社

MDPI
DOI: 10.3390/ijms22094890

关键词

redox balance; NADH shuttle; peroxisomal malate dehydrogenase; environment-dependent peroxisomal targeting; intraorganellar protein crowding

资金

  1. NIH [DK41737]

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This study investigates the localization of NADH-shuttling proteins in Pichia pastoris and introduces a novel BiFC variant called divergent BiFC for detecting colocalization of noninteracting proteins. The findings demonstrate a partial peroxisomal location of a specific NADH-shuttling protein only under certain growth conditions, shedding light on the medium-dependent compartmentalization of proteins in the yeast.
The maintenance of intracellular NAD(+)/NADH homeostasis across multiple, subcellular compartments requires the presence of NADH-shuttling proteins, which circumvent the lack of permeability of organelle membranes to these cofactors. Very little is known regarding these proteins in the methylotrophic yeast, Pichia pastoris. During the study of the subcellular locations of these shuttling proteins, which often have dual subcellular locations, it became necessary to develop new ways to detect the weak peroxisomal locations of some of these proteins. We have developed a novel variation of the traditional Bimolecular Fluorescence Complementation (BiFC), called divergent BiFC, to detect intraorganellar colocalization of two noninteracting proteins based on their proximity-based protein crowding within a small subcellular compartment, rather than on the traditional protein-protein interactions expected for BiFC. This method is used to demonstrate the partially peroxisomal location of one such P. pastoris NADH-shuttling protein, malate dehydrogenase B, only when cells are grown in oleate, but not when grown in methanol or glucose. We discuss the mode of NADH shuttling in P. pastoris and the physiological basis of the medium-dependent compartmentalization of PpMdhB.

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