期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/ijms22094913
关键词
fiber type; glucose transporter; insulin signaling; myosin heavy chain; type 2 diabetes
资金
- National Institute of Diabetes and Digestive and Kidney Diseases [R01DK103562, R01DK043051]
- National Institute of Arthritis and Musculoskeletal and Skin Diseases [R01AR070200]
Denervation induces insulin resistance in skeletal muscle, with the soleus muscle showing sustained resistance and the EDL muscle showing enhanced insulin sensitivity due to increased GLUT4 protein levels. Insulin-stimulated glucose uptake was significantly altered in both muscles post-denervation, showcasing differential effects in oxidative and glycolytic muscle types.
Y Denervation rapidly induces insulin resistance (i.e., impairments in insulin-stimulated glucose uptake and signaling proteins) in skeletal muscle. Surprisingly, whether this metabolic derangement is long-lasting is presently not clear. The main goal of this study was to determine if insulin resistance is sustained in both oxidative soleus and glycolytic extensor digitorum longus (EDL) muscles following long-term (28 days) denervation. Mouse hindlimb muscles were denervated via unilateral sciatic nerve resection. Both soleus and EDL muscles atrophied similar to 40%. Strikingly, while denervation impaired submaximal insulin-stimulated [3H]-2-deoxyglucose uptake similar to 30% in the soleus, it enhanced submaximal (similar to 120%) and maximal (similar to 160%) insulin-stimulated glucose uptake in the EDL. To assess possible mechanism(s), immunoblots were performed. Denervation did not consistently alter insulin signaling (e.g., p-Akt (Thr308):Akt; p-TBC1D1 [phospho-Akt substrate (PAS)]:TBC1D1; or p-TBC1D4 (PAS):TBC1D4) in either muscle. However, denervation decreased glucose transporter 4 (GLUT4) levels similar to 65% in the soleus but increased them similar to 90% in the EDL. To assess the contribution of GLUT4 to the enhanced EDL muscle glucose uptake, muscle-specific GLUT4 knockout mice were examined. Loss of GLUT4 prevented the denervation-induced increase in insulin-stimulated glucose uptake. In conclusion, the denervation results sustained insulin resistance in the soleus but enhanced insulin sensitivity in the EDL due to increased GLUT4 protein levels.
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