期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/ijms22083961
关键词
placenta; preeclampsia; trophoblast; miR-210-3p; CDX2; cytokines; interleukin-1B; interleukin-8; CXCL1
资金
- Canadian Institutes of Health Research [CIHR PJT-153146]
- Canada Foundation for Innovation/Ontario Research Fund [35611]
- York University Research Chair Program
- CIHR [CCI-132565, FDN-143262]
- Ontario Graduate Scholarship
- Natural Sciences and Engineering Research Council of Canada (NSERC)
The overexpression of miR-210-3p reduces trophoblast migration and invasion, affects EVT outgrowth in early pregnancy explants, and decreases the ability of trophoblast to form endothelial-like networks. Furthermore, miR-210-3p downregulates the expression of certain cytokines, potentially influencing EVT invasion and immune cell recruitment.
Hsa-miR-210-3p has been reported to be upregulated in preeclampsia (PE); however, the functions of miR-210-3p in placental development are not fully understood, and, consequently, miR-210-3p's role in the pathogenesis of PE is still under investigation. In this study, we found that overexpression of miR-210-3p reduced trophoblast migration and invasion, extravillous trophoblast (EVT) outgrowth in first trimester explants, expression of endovascular trophoblast (enEVT) markers and the ability of trophoblast to form endothelial-like networks. In addition, miR-210-3p overexpression significantly downregulated the mRNA levels of interleukin-1B and -8, as well as CXC motif ligand 1. These cytokines have been suggested to play a role in EVT invasion and the recruitment of immune cells to the spiral artery remodeling sites. We also showed that caudal-related homeobox transcription factor 2 (CDX2) is targeted by miR-210-3p and that CDX2 downregulation mimicked the observed effects of miR-210-3p upregulation in trophoblasts. These findings suggest that miR-210-3p may play a role in regulating events associated with enEVT functions and its overexpression could impair spiral artery remodeling, thereby contributing to PE.
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