Quality characteristics of high salt fermented fish sauce (budu) produced using autochthonous Virgibacillus halodenitrificans PS21 and Staphylococcus simulans PMRS35

This study evaluated the effects of autochthonous starters on the quality characteristics of budu. The effects of individual or combined inoculation of Virgibacillus halodenitrificans PS21 and Staphylococcus simulans PMRS35 were compared to those of non-inoculation during 150 days of budu production. The starters directly affected characteristics of budu with browner colour and enhanced proteolysis and lipolysis as indicated by degree of hydrolysis and free fatty acid contents. The combined starter inoculation resulted in a high aspartic acid, glutamic acid and lysine levels. Additionally, key desirable volatiles, including 2-methylbutanal, 3-methylbutanal, benzaldehyde and 2-ethyl furan, were dominant in the starter-inoculated budu. In a sensorial analysis, the highest scores for appearance, colour, taste and flavour were assigned for inoculated budu with combined starters. Therefore, the addition of starters (V. halodenitrificans PS21 and S. simulans PMRS35) in budu production improved the quality characteristics and reduced the fermentation period.

Automated summary

The addition of combined starters Virgibacillus halodenitrificans PS21 and Staphylococcus simulans PMRS35 significantly improved the quality characteristics of budu, including increased proteolysis and lipolysis, enriched amino acid content, and higher levels of key volatile compounds in the fermented product.