Development of a D-μSPE method based on curcumin-modified magnetic reduced graphene oxide nanocomposite for the determination of Trichostatin A in a biological sample

In the current work, a novel curcumin-modified magnetic graphene oxide nanocomposite was synthesised through a facile method and the loading of Trichostatin A as an anticancer drug was evaluated. D-mu SPE method coupled with high-performance liquid chromatography (HPLC-DAD) successfully applied to determine the target analyte. The proper synthesis of the nanocomposite was investigated using Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy and vibrating sample magnetometry. The Taguchi statistical design was used to investigate the impact of five operating variables. The results for signal/noise ratio (S/N) and analysis of variance showed that the MG-curcumin/TSA ratio was the most significant factor while the solution pH was the least significant variable. In addition, the optimum operating conditions were obtained and adsorption isotherms were also studied. The optimised parameters were employed to perform extraction of the TSA from real samples. Good linearity (with r (2) = 0.9950) was shown for TSA concentrations from 5.0 to 1000.0 ng mL(-1). Moreover, detection limit (0.70 ng mL(-1)), limit of quantification (2.51 ng mL(-1)), Inter-day and intra-day precision (n = 5, 3.2%, and 5.0%) were achieved for the method.

Automated summary

In this study, a novel curcumin-modified magnetic graphene oxide nanocomposite was synthesized and evaluated as a carrier for an anticancer drug. The optimal operating conditions were determined and the performance of the method was assessed through various experiments and analyses.