期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 177, 期 -, 页码 317-327出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.02.131
关键词
C-kit(+) hematopoietic stem cells; Natural killer cells; Alginate-gelatin encapsulation; Telomere length; Cytokines; Janus kinase/signal transducers and activators of transcription signaling pathway
资金
- National Institute for Medical Research Development, Iran [972433]
The study investigated the effect of alginate-gelatin encapsulation on the differentiation potential of NK cells, revealing that encapsulation increased cell apoptosis and telomere length, as well as promoted NK cell differentiation through the secretion of cytokines.
The ability of natural killer (NK) cells to destroy cancerous cells with no prior sensitization has made them attractive candidates for cell therapy. The application of hydrogels must be notified as cell delivery vehicles in cell differentiation. The present study was conducted to investigate the effect of alginate-gelatin encapsulation on NK cell differentiation potential of C-kit(+) cells. C-kit(+) cells were differentiated to NK cells under both encapsulated and un-encapsulated conditions. Next, the cells were subjected to real-time polymerase chain reaction (PCR) and western blotting for the assessment of their telomere length and protein expressions, respectively. Afterward, culture medium was collected to measure cytokines levels. Thereafter, the differentiated NK cells were co cultured with Molt-4 cells to investigate the potency of cell apoptosis by Annexin V/PI assay. A significant change was observed in the protein expression of Janus kinase/Signal transducers (JAK/STAT) pathway components. Additionally, the encapsulation caused an increase in the apoptosis of Molt-4 cells and telomere length of NK cells differentiated C-kit(+) cells. Therefore, it can be concluded that the effects of encapsulation on NK cell's differentiation of C-kit(+) cells could be resulted from the secreted cytokines of interleukin (IL)-2, IL-3, IL-7, and IL-12 as well as the increased telomere length. (c) 2021 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据