期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 178, 期 -, 页码 94-103出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.02.046
关键词
Carboxymethyl pachymaran; Structure; Chain conformation; Immunomodulatory
资金
- National Key Research and Development Plan of China [2017YFC1703006]
- Agricultural Science and Technology Innovation Center of Hubei Province [2016620000001044]
Carboxymethyl pachymaran (CMP) was prepared from Poria cocos polysaccharide by carboxymethylation, resulting in two types of CMP (CMP-1 and CMP-2) with immunomodulatory activities. Both CMP-1 and CMP-2 contain a beta-(1 -> 3)-D-glucan structure that can promote macrophage activity, with CMP-1 exhibiting stronger immunomodulatory ability.
Carboxymethyl pachymaran (CMP) was prepared from Poria cocos polysaccharide by carboxymethylation. Two types of CMP(CMP-1 and CMP-2) were further purified byDEAE-52 anion-exchange chromatography. The structure characteristics and immunomodulatory activities of CMP-1 and CMP-2were investigated. CMP-1was determined as beta-(1 -> 3)-D-glucan. A beta-(1 -> 3)-D-glucan backbone structure was also found in CMP-2, which was mainly consistent of mannose and glucose, with the mole ratio of 0.03:1. The molecular weight of CMP-1 was 126.1 kDa with a 30.4 nm irregular sphere in distilled water. However, the molecular weight of CMP-2 was 172.6 kDa in a 19.9 nm spherical structure in water solution. Both CMP-1 and CMP-2 had triple helical structure, which can promote the proliferation and the phagocytosis of macrophages. Moreover, CMP-1 and CMP-2 both could improve the secretions of NO, TNF-alpha and IL-6 by increasing the expression of iNOS, TNF-alpha and IL-6 mRNA, but CMP-1 exhibited a stronger immunomodulatory ability than that of CMP-2. Our results indicated that CMP-1 and CMP-2 can act as potential immunomodulatory agents. (C) 2021 Elsevier B.V. All rights reserved.
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