Characterization of Anoectochilus roxburghii polysaccharide and its therapeutic effect on type 2 diabetic mice

Anoectochilus roxburghii is a traditional herb in China that can be potentially used to treat diabetes. A novel polysaccharide ARLP-Wwas isolated fromAnoectochilus roxburghii by chromatography on DEAE-52 cellulose. Chemical analysis indicated that ARLP-W (8.1 x 10(4) Da) was mainly composed of mannose and glucose. The main linkages of glycosidic bonds of ARLP-W were beta-1, 4-Manp and alpha-1, 4-Glcp. The terminal Glcp was connected to Manp-via O-3. RT-qPCR and western blotting analysis showed that ARLP-W caused a significant reduction in the levels of the key gluconeogenesis enzymes phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver. The results of the insulin resistance tests indicated that ARLP-W increased glucose absorption. These results indicate that ARLP-W has a good therapeutic effect on type 2 diabetes and can assist with further development and application treatment of diabetes. (C) 2018 Published by Elsevier B.V.

Automated summary

ARLP-W, a polysaccharide extracted from Anoectochilus roxburghii, mainly composed of mannose and glucose, showed significant reduction in gluconeogenesis enzymes in the liver and increased glucose absorption. These results suggest that ARLP-W may have a good therapeutic effect on type 2 diabetes.