Discrimination of conspecifics from heterospecifics in a hybrid zone: Behavioral and chemical cues in ants

Species and nestmate recognition in social insects occurs mostly through cuticular hydrocarbons acting as chemical cues. These compounds generate a colony-specific odor profile depending on genetic and environmental factors. Species and nestmate recognition results in specific behavioral responses, regulating the level of aggression toward other individuals during an interaction. Although species discrimination and recognition cues have been poorly studied in the context of interspecific hybridization, such systems offer an opportunity to further investigate the influence of heritable and environmental factors on recognition. We explored the strength of discrimination in a hybrid zone between two ant species-Tetramorium immigrans and T. caespitum-by comparing cuticular hydrocarbon profiles and measuring intra- and interspecific worker aggression in both areas of sympatry and areas of allopatry among species. Species cuticular hydrocarbon profiles were well-differentiated and interspecific aggression was high, revealing highly discriminating species recognition cues. Hybrids' cuticular hydrocarbon profiles consisted of a mixture of the parental bouquets, but also exhibited hybrid-specific patterns. Behavioral assays showed that T. immigrans is as aggressive toward hybrids as toward heterospecifics. Finally, aggression between heterospecific workers was lower when interacting individuals came from areas of sympatry among species than from areas of allopatry. Taken as a whole, these findings paint a particularly complex picture of the recognition system in T. immigrans, T. caespitum, and their hybrids, and highlight that hybrid zones afford a still underexplored opportunity for investigating recognition mechanisms and discrimination between species.

Automated summary

Species and nestmate recognition in social insects mainly relies on cuticular hydrocarbons, which generate colony-specific odor profiles and regulate aggression. Hybrid zones between ant species show well-differentiated hydrocarbon profiles and high interspecific aggression, with hybrids displaying a mixture of parental and unique patterns. Interactions between heterospecific workers vary based on whether they come from sympatric or allopatric areas, emphasizing the complexity of recognition mechanisms and discrimination in hybrids.