期刊
IMMUNOLOGY
卷 163, 期 4, 页码 436-447出版社
WILEY
DOI: 10.1111/imm.13327
关键词
B cell; CD22; human; immune regulation; red blood cell; sialic acid
类别
资金
- Cancer Research Aberdeen North-East Scotland (CRANES)
- Wellcome Trust [094847]
Non-immune cells, specifically red blood cells, play a role in regulating the activation state of human B cells by inhibiting their activation through contact and involving sialic acids and the CD22 receptor. This novel mechanism may contribute to suppressing inappropriate responsiveness of healthy B cells in the bloodstream.
Non-immune cells are increasingly recognized as important in regulating immunity, but the role of red blood cells (RBC) remains relatively unexplored, despite their abundance in the circulation and a cell surface rich in potential ligands. Here, we determine whether RBC influence the activation state of human B cells. Separation of RBC from peripheral blood mononuclear cells increased B-cell expression of HLA-DR/DP/DQ, whilst reconstitution reduced the levels of B-cell activation markers HLA-DR/DP/DQ, CD86, CD69 and CD40, as well as decreasing proliferative responses and IgM secretion. Inhibition of B cells required contact with RBC and was abrogated by either removal of sialic acids from RBC or blocking the corresponding lectin receptor CD22 on B cells. Chronic lymphocytic leukaemia B cells express low levels of CD22 and were less susceptible to inhibition by RBC, which may contribute to their activated phenotype. Taken together, the results identify a novel mechanism that may suppress inappropriate responsiveness of healthy B cells whilst circulating in the bloodstream.
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